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Research Compound CatalogRecovery & Regenerative ResearchTB-500 Research Profile
Recovery & Regenerative Research
Research Use Only

Synthetic Thymosin Beta-4 Actin-Binding Domain Fragment

TB-500

Thymosin Beta-4 Actin-Binding Domain Fragment

Research StatusLiterature Reviewed Through: July 2026
Research Use Only
Updated Periodically

Research Profiles are reviewed periodically as new peer-reviewed literature becomes available.

Available Strength
5 mg / vial
CAS Number
77591-33-4
Recovery & Regenerative Research laboratory research
Recovery & Regenerative Research
Research at a Glance

Major published findings — summarized in under 30 seconds.

1
Enhanced cell migration in wound healing models has been consistently observed in laboratory studies.
2
Promotion of new blood vessel formation has been reported in endothelial cell and in vivo models.
3
Support for muscle satellite cell activation — relevant to skeletal muscle repair — has been observed.
4
Reductions in inflammatory markers have been reported in tissue repair contexts.
5
The compound has been well-characterized in standard scratch wound and tube formation assay models.

What Is This Compound?

TB-500 is a synthetic peptide that corresponds to a specific region of a naturally occurring protein called Thymosin Beta-4, which is found in high concentrations in blood platelets and wound fluid throughout the body. Thymosin Beta-4 plays an important role in how cells move and reorganize during tissue repair, and TB-500 is studied to understand the specific contribution of this region to those processes. Researchers have used it to investigate wound healing, muscle repair, and the formation of new blood vessels. Its smaller size compared to the full protein makes it practical for laboratory and animal research.

Continue below to see what published research has observed so far.

What Has Research Found?

1
Enhanced cell migration in wound healing models has been consistently observed in laboratory studies.
2
Promotion of new blood vessel formation has been reported in endothelial cell and in vivo models.
3
Support for muscle satellite cell activation — relevant to skeletal muscle repair — has been observed.
4
Reductions in inflammatory markers have been reported in tissue repair contexts.
5
The compound has been well-characterized in standard scratch wound and tube formation assay models.

Want to understand why these findings occur? Continue to the scientific overview below.

The Science Behind It

TB-500 is a synthetic peptide corresponding to the actin-binding domain of Thymosin Beta-4 (Tβ4), a naturally occurring 43-amino acid protein found in high concentrations in blood platelets, wound fluid, and various tissues. Thymosin Beta-4 plays a central role in actin sequestration and cytoskeletal dynamics, and TB-500 is studied as a research tool to investigate the regenerative and tissue-protective properties attributed to this region of the full-length protein.

Mechanism of Action

TB-500 promotes actin polymerization and cell migration by sequestering G-actin monomers, facilitating cytoskeletal reorganization essential for wound healing and tissue repair. Research indicates it upregulates cell surface receptors involved in migration, promotes angiogenesis through endothelial cell activation, and modulates inflammatory responses. The peptide has been studied for its ability to promote satellite cell activation in muscle tissue and support extracellular matrix remodeling.

Key Research Characteristics
Corresponds to the actin-binding domain (LKKTETQ region) of full-length Thymosin Beta-4
Promotes G-actin sequestration and cytoskeletal reorganization critical for cell migration
Studied for angiogenic effects through endothelial cell activation and VEGF modulation
Investigated for muscle satellite cell activation and skeletal muscle repair biology
Smaller molecular weight than full-length Tβ4 — practical for cell penetration and in vivo studies
Well-characterized in scratch wound migration and tube formation assay models

Research Applications

Actin dynamics and cytoskeletal biology research
Wound healing and tissue repair models
Angiogenesis and endothelial cell biology
Muscle satellite cell activation studies
Inflammatory resolution and tissue remodeling research

Compound Specifications

Available Strength
5 mg / vial
Appearance
White lyophilized powder
Purity
≥98% (HPLC)
Storage Conditions
−20 °C; protect from light
Storage After Reconstitution
4 °C up to 7 days; −80 °C for long-term storage
Molecular Formula
C₂₁₂H₃₅₀N₅₆O₇₈S
Molecular Weight
4963.5 g/mol
CAS Number
77591-33-4
Research Status
Research Use Only (RUO)

Scientific References

1.Goldstein AL, et al. Thymosin beta4: a multi-functional regenerative peptide. Basic properties and clinical applications. Expert Opin Biol Ther. 2012;12(1):37–51.
2.Bock-Marquette I, et al. Thymosin beta4 activates integrin-linked kinase and promotes cardiac cell migration, survival and cardiac repair. Nature. 2004;432(7016):466–472.
3.Huff T, et al. beta-Thymosins, small acidic peptides with multiple functions. Int J Biochem Cell Biol. 2001;33(3):205–220.
4.[Placeholder] Peer-reviewed publication on TB-500 satellite cell activation in skeletal muscle models.
5.[Placeholder] Technical reference: stability and bioactivity validation data for TB-500.

Placeholder entries are reserved for peer-reviewed publications. All references are provided for scientific context only.

Current State of Research

Research Use Only

All information presented on this Research Profile is intended exclusively for laboratory research purposes. This compound is designated Research Use Only (RUO) and is not intended for human consumption, veterinary use, or any clinical application. It is not intended to diagnose, treat, cure, or prevent any disease or medical condition.

This material is sold solely for in vitro research and laboratory investigation by qualified scientific personnel. Kali Research LLC makes no representations regarding the safety or efficacy of this compound for any use outside of controlled laboratory research settings. Researchers are solely responsible for compliance with all applicable laws and institutional regulations governing the use of research compounds.

Not for human consumptionNot for veterinary useNot for clinical applicationQualified researchers onlyIn vitro / laboratory use
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Research Profile
Specifications
Available Strength5 mg / vial
AppearanceWhite lyophilized powder
Purity≥98% (HPLC)
Storage Conditions−20 °C; protect from light
Storage After Reconstitution4 °C up to 7 days; −80 °C for long-term storage
Molecular FormulaC₂₁₂H₃₅₀N₅₆O₇₈S
Molecular Weight4963.5 g/mol
CAS Number77591-33-4

Need additional technical documentation? Submit an inquiry and our team will respond.

Research Use Only (RUO). Not for human consumption, veterinary use, or clinical application.